Journal: iScience

Article Title: Favine/CCDC3 deficiency accelerated atherosclerosis and thrombus formation is associated with decreased MEF2C-KLF2 pathway

doi: 10.1016/j.isci.2022.105252

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Article Snippet: PAI-1 total antigen ELISA kit , Molecular Innovations , Cat #MPAIKT-TOT.

Techniques: Recombinant, Transfection, Staining, Sample Prep, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Software

Journal: Pharmaceutical research

Article Title: A Role for Low Density Lipoprotein Receptor-related Protein 1 in the Cellular Uptake of Tissue Plasminogen Activator in the Lungs

doi: 10.1007/s11095-015-1763-6

Figure Lengend Snippet: The concentration of intracellular fraction mtPA was lower over time in the presence of LRP1 inhibitor, receptor associated protein (RAP) in primary liver and lung cell suspensions (20,000 cells each). Cells were exposed to mtPA (50ng) at time 0 in the presence or absence of RAP (400nM). Representative Western blots of intracellular fraction mtPA over time in (A) primary whole liver cell lysates and (B) primary whole lung cell lysates. These findings were corroborated by the measurement of intracellular fraction mtPA concentrations measured by ELISA in (C) liver cell (*p=0.0132 at 1 min) and (D) lung cell (*p=0.009; +p<0.001; #p=0.066) lysates as well as in extracellular fraction (supernatant) media of (E) liver (*p=0.005; +p=0.0005) and (F) lung (*p=0.024; +p=0.001) cell suspensions. Data are the mean (±S.E.M.) of 3-4 samples/group and p values were generated from post ANOVA Holm-Sidak tests.

Article Snippet: Measurement of mtPA Uptake by Lung and Liver Cell Suspensions The concentration of mtPA in lung and liver cell lysates (intracellular fraction) and supernatants (extracellular fraction) was detected using mtPA total antigen assay ELISA kit (MTPAKT-TOT, Molecular Innovations).

Techniques: Concentration Assay, Western Blot, Enzyme-linked Immunosorbent Assay, Generated

Journal: Stroke

Article Title: Blood vitronectin induces detrimental brain IL-6 and correlates with outcomes after stroke only in female mice

doi: 10.1161/STROKEAHA.120.029036

Figure Lengend Snippet: A) Plasma VTN concentrations were increased 24 hours after compared to 24 hours before MCAO in female, but not male, VTN+/+ (N=10,7,10,12) and C57BL/6 mice (N=13,11). B) At 7 days, plasma VTN returned to baseline levels in females, but MCAO did not change plasma VTN in males (N= 6,7, repeated measures). C) Plasma VTN concentrations in female, but not male, C57BL/6 mice at 24 hours after MCAO correlated to injury size at 7 days, measured in GFAP-stained sections (N=6,7). D) MCAO increased plasma VTN at 24 hours compared to both naïve and sham operated C57BL/6 female mice (N=6,7,4). * p<0.05, ** p<0.01, *** p<0.001, **** or #### p<0.0001

Article Snippet: Plasma VTN concentrations were measured by ELISA (MVNKT-TOT, Molecular Innovations) and in the injured striatum by western blot.

Techniques: Staining

Journal: Stroke

Article Title: Blood vitronectin induces detrimental brain IL-6 and correlates with outcomes after stroke only in female mice

doi: 10.1161/STROKEAHA.120.029036

Figure Lengend Snippet: At 14 days after MCAO, VTN−/− female mice showed less A) CD68-positive activated microglia/macrophages and B) CD45-positive infiltrated leukocytes than VTN+/+ females, as shown by area and density measurements (N=6,5; * p<0.05, ** p<0.01). Dashed lines delineate injured areas with dense immunostained cells. C) Plasma VTN concentrations in female C57BL/6 mice at 24 hours correlated to CD68 and CD45 density at 7 days after MCAO (N=6). D) No correlation was present in males (N=7).

Article Snippet: Plasma VTN concentrations were measured by ELISA (MVNKT-TOT, Molecular Innovations) and in the injured striatum by western blot.

Techniques:

Journal: Stroke

Article Title: Blood vitronectin induces detrimental brain IL-6 and correlates with outcomes after stroke only in female mice

doi: 10.1161/STROKEAHA.120.029036

Figure Lengend Snippet: A) In VTN−/− females, MCAO-induced IL-6 in the injured striatum at 24 hours after MCAO was much less compared to VTN+/+ females. There were no genotype differences in MCAO-induced ciliary neurotrophic factor (CNTF) or leukemia inhibitory factor (LIF). N=5,7,6,6, * or # p<0.05, ** p<0.01. B) Males did not show genotype differences in MCAO-induced IL-6, CNTF or LIF (N=6,10,8,11). Plasma VTN concentrations correlated with IL-6, CNTF and LIF expression in females C), but not males D), N=11/group.

Article Snippet: Plasma VTN concentrations were measured by ELISA (MVNKT-TOT, Molecular Innovations) and in the injured striatum by western blot.

Techniques: Expressing